hplc测三七中皂苷，三七皂苷注射制剂的功效和作用

发布时间：2023-05-31 09:17 编辑：网络点击：193

本文目录一览三七皂苷注射制剂的功效和作用2，三七总皂苷液相色谱出峰顺序3，三七总皂苷的药物应用4，三七皂苷的研究论文5，HPLC测试6，三七皂苷是一种口服药物最好使用什么来提取7，求助药动学HPLC荧光法检测8，田七和三七有什么区别9，……

本文目录一览

1，三七皂苷注射制剂的功效和作用
2，三七总皂苷液相色谱出峰顺序
3，三七总皂苷的药物应用
4，三七皂苷的研究论文
5，HPLC测试
6，三七皂苷是一种口服药物最好使用什么来提取
7，求助药动学HPLC荧光法检测
8，田七和三七有什么区别
9，欧洲越橘美国药典HPLC测定方法是怎样的

1，三七皂苷注射制剂的功效和作用

主要用来治疗心血管方面的疾病，对脏器出血以及人体凝血功能也有作用！

hplc测三七中皂苷

2，三七总皂苷液相色谱出峰顺序

反相液相色谱法中,物质极性强的先出峰,极性弱的后出峰,在正相色谱中相反,物质极性强的后出峰,极性弱的先出峰。当然这个不是完全固定的。具体的看你的实验方法,看固定相和流动相。一般反相色谱是极性大的先出峰,极性小的后出峰。不过如果流动相中有酸碱性就不一定了。比如流动相是弱酸性的,那么样品中的碱性物质会提前出峰。

hplc测三七中皂苷

3，三七总皂苷的药物应用

三七总皂苷目前主要用应用心脑血管疾病类的药品、保健品等如“血塞通”“复方丹参滴丸”

hplc测三七中皂苷

4，三七皂苷的研究论文

学术论文在形式上是属于议论文的，但它与一般议论文不同，它必须是有自己的理论系统的，不能只是材料的罗列，应对大量的事实、材料进行分析、研究，使感性认识上升到理性认识。一般来说，学术论文具有论证色彩，或具有论辩色彩。论文的内容必须符合历史唯物主义和唯物辩证法，符合“实事求是”、“有的放矢”、“既分析又综合” 的科学研究方法。

5，HPLC测试

线性检测器有一定的线性范围，不在范围内的测定误差很大，可能不成线性。还有些检测器的响应和浓度不是线性的，例如蒸发散光检测器，峰面积和和浓度成对数关系觉得对给个满意嘛，谢谢！

6，三七皂苷是一种口服药物最好使用什么来提取

冻死

一种从中药三七中提取纯化总皂苷的制备方法，通过将三七药材粉碎成过粉末或颗粒，去除杂质，烘干，用含浓度为0-95％的乙醇水溶媒渗滤，热回流提取，提取液减压浓缩富集，将浓缩液上D101大孔树脂，依次以水、氨水、醇水洗脱，收集洗脱液，浓缩，干燥，即得三七总皂苷精制物。本发明的方法制成的三七总皂苷，可制成片剂、胶囊、注射剂等多种药剂学上所说的剂型，也可配合其它药物或组分一起制成制剂使用。本发明方法只需乙醇水溶液提取，使用一次大孔树脂柱分离，终产物三七总皂苷纯度高，产品稳定性和均一性良好。本发明方法设计合理，低成本、易操作、稳定性好、质控更严格、易于产业化生产。

7，求助药动学HPLC荧光法检测

我记得可以用丹磺酰氯进行衍生化反应，之后在用紫外或荧光检测器来做。之前没有做过，请教几个问题：1、丹磺酰氯用什么来溶解?一般浓度是多少？2、因为做的是大鼠的药动，那么是先将药物从血浆中提取出来，在进行衍生反应，还是直接将一定量的丹磺酰氯溶液加入到血浆中衍生呢？（个人觉得是先提取，再衍生）3、用丹磺酰氯时，用PH在什么范围的缓冲溶液？（文献用来与生物胺衍生时，都是用碱性环境）以及用什么缓冲液？4、用丹磺酰氯时，衍生反应要避光吗？（文献，有的要，有的没有提是否避光）备注：我的药物有两个仲胺

自己先发表一下,可否用HPLC-蒸发光散射检测器来做,有资料显示,一来是通用型检测器,二来它可以解决HPLC-紫外检测器,所难以解决的困难:对于皂苷,糖类等五紫外吸收或末端吸收及紫外吸收吸收系数很小的化合物.而且它的检测限也可以达到ng级

8，田七和三七有什么区别

一种草药的两个名字田七和和三七是一种草药的两个名字，可能大家比较耳熟能详的是田七，但是三七才是它的“大名”，而“田七”更像是它的小名。叫法根据地区不同也有所不同，文山地区的人大多叫三七，其他地方的人叫大多田七，都是五加科人参属的植物。田七和和三七是一种草药的两个名字。名字由来：古时候，有一个姓张的年轻人嘴里总是流血。正巧田医生路过，用草药给小张止血，效果还不错。于是，小张问田医生要到了这种草药的种子种在自家院子里。第二年药草长出来之后，发现果然长的很好。这时，知府大人贴出告示，知府大人的千金也得了类似得病，若有人能够治好，还会把千金许配给他。小张一看，有一种正中下怀的得意瞬间涌上心头，赶紧把药送给知府大人。可没想到的是，大人的千金死了！知府捉住了小张和田医生。田医生解释道，那是因为这种草药只能在三年到七年的时候有效啊！田医生当场割坏自己的大腿，又用自带药粉涂抹在伤口上，立刻止血。知府无奈，放了田医生。因此，三七的意思是这种草药三到七年才能使用。田七粉的功效与作用：1、保护心脑血管，田七粉是人类心脑血管的保护神，这种食材既能活血也能养血，它能增加血管弹性防止血管老化僵化，还能净化血液加快身体代谢，它对人类的血栓动脉硬化以及血栓等症都有明显预防作用。2、补益气血，田七粉是一种功效出色的补血食材，它能提高人体造血功能让人体气血亏损的症状，尽快缓解，另外它还能起到活血美容的重要作用，它对人类经常出现的面色黯淡无光和面色暗黄以及色斑等多种不良症状，都有明显调理作用，能让人们的面色变得红润健康。3、延缓衰老，田七粉中含有一些天然皂苷和多糖，还含有多种天然活性成分，人体吸收这些物质以后，身体各个器官的组织细胞活性都能明显提高，而且它能增强脑细胞中sod的活性，能让人体各器官都保持正常年轻的状态，这种情况下身体的衰老速度就会放慢很多。田七粉的食用方法：1、田七粉最常见的使用方法可以分为生吃和熟吃两种，生吃就是指把田七研磨成粉末状以后直接食用，每次的服用量应该在5到6克之间，每天服用2到3次，这时的田七粉味道比较苦，但它预防高血脂和活血化瘀的功效却特别出色。2、田七粉除了可以直接吃以外，还可以制成多种药膳供人们食用，人们在炖肉或者炖排骨以及炖鸡时，都可以加入适量的田七粉，把它制成药膳，然后再吃肉喝汤，这样就能让身体尽快把田七粉含有的营养吸收和利用。把田七粉制成药膳以后食用，有利于它补血功效的发挥。

9，欧洲越橘美国药典HPLC测定方法是怎样的

Content of anthocyanosides and anthocyanidins— Solvent: a mixture of methanol and hydrochloric acid (98:2). Diluent: a mixture of water and 85% phosphoric acid (9:1). Solution A— Prepare a filtered and degassed mixture of water and formic acid (9:1). Solution B— Prepare a filtered and degassed mixture of water, acetonitrile, methanol, and formic acid (40:22.5:22.5:10). Mobile phase— Use variable mixtures of Solution A and Solution B as directed for Chromatographic system. Make adjustments if necessary (see System Suitability under Chromatography 621). Standard solution 1— Dissolve, using sonication, an accurately weighed quantity of USP Cyanidin-3-O-glucoside Chloride RS in Solvent to obtain a solution having a known concentration of about 0.4 mg per mL. Transfer 2.0 mL of this solution to a 10-mL volumetric flask, dilute with Diluent to volume, and mix. [note—This solution is stable for 48 hours at 4.] Standard solution 2— Dissolve, using sonication, an accurately weighed quantity of USP Cyanidin Chloride RS in Solvent to obtain a solution having a known concentration of about 0.5 mg per mL. Transfer 2.0 mL of this solution to a 100-mL volumetric flask, dilute with Diluent to volume, and mix. [note—This solution is stable for 36 hours at 4.] Standard solution 3— Transfer about 125 mg of USP Powdered Bilberry Extract RS, accurately weighed, to a 100-mL volumetric flask, add 25 mL of Solvent, sonicate to dissolve, dilute with Diluent to volume, and mix. [note—This solution is stable for 48 hours at 4.] Test solution— Proceed as directed for Standard solution 3, except to use Powdered Extract. Chromatographic system (see Chromatography 621)— [note—Use deactivated silanized HPLC vials.] The liquid chromatograph is equipped with a refrigerated autosampler maintained at 4, a 535-nm detector, and a 4.6-mm × 25-cm column that contains 5-μm L1 packing. The flow rate is about 1.0 mL per minute. The column temperature is maintained at 30 ± 1. The chromatograph is programmed as follows: Time(minutes) Solution A (%) Solution B (%) Elution 0–35 93?75 7?25 linear gradient 35–45 75?35 25?65 linear gradient 45–46 35?0 65?100 linear gradient 46–50 0 100 isocratic 50–51 0?93 100?7 linear gradient 51–60 93 7 isocratic Chromatograph Standard solution 1, and record the peak responses as directed for Procedure: the tailing factor for the cyanidin-3-O-glucoside chloride peak is not less than 0.8 and not more than 2.0; and the relative standard deviation determined from the cyanidin-3-O-glucoside chloride peak for replicate injections is not more than 2.0%. Chromatograph Standard solution 3, and record the peak responses as directed for Procedure: the chromatogram obtained is similar to the Reference chromatogram provided with the lot of USP Powdered Bilberry Extract RS being used; the resolution, R, for the delphinidin-3-O-arabinoside, malvidin-3-O-galactoside, and petunidin-3-O-arabinose peaks is not less than 0.8; and the resolution, R, for other components is not less than 1.0. Procedure— Separately inject equal volumes (about 10 μL) of Standard solution 1, Standard solution 2, Standard solution 3, and the Test solution into the chromatograph; record the chromatograms; and measure the areas of the analyte peaks. Using the chromatogram of Standard solution 3 and the Reference chromatogram provided with the lot of USP Powdered Bilberry Extract RS being used, identify the retention times of the peaks corresponding to the different anthocyanosides and anthocyanidins. The approximate relative retention times of the anthocyanosides and anthocyanidins are provided in the following table: Analyte Relative RetentionTime Delphinidin-3-O-galactoside chloride 0.61 Delphinidin-3-O-glucoside chloride 0.73 Cyanidin-3-O-galactoside chloride 0.84 Delphinidin-3-O-arabinoside chloride 0.86 Cyanidin-3-O-glucoside chloride 1.00 Petunidin-3-O-galactoside chloride 1.08 Cyanidin-3-O-arabinoside chloride 1.11 Petunidin-3-O-glucoside chloride 1.24 Delphinidin chloride 1.28 Peonidin-3-O-galactoside chloride 1.36 Petunidin-3-O-arabinoside chloride 1.39 Peonidin-3-O-glucoside chloride 1.55 Malvidin-3-O-galactoside chloride 1.58 Peonidin-3-O-arabinoside chloride 1.67 Malvidin-3-O-glucoside chloride 1.76 Cyanidin chloride 1.82 Malvidin-3-O-arabinoside chloride 1.91 Petunidin chloride 2.08 Peonidin chloride 2.27 Malvidin chloride 2.30 Separately calculate the percentages of delphinidin-3-O-galactoside chloride, delphinidin-3-O-glucoside chloride, cyanidin-3-O-galactoside chloride, delphinidin-3-O-arabinoside chloride, cyanidin-3-O-glucoside chloride, petunidin-3-O-galactoside chloride, cyanidin-3-O-arabinoside chloride, petunidin-3-O-glucoside chloride, peonidin-3-O-galactoside chloride, petunidin-3-O-arabinoside chloride, peonidin-3-O-glucoside chloride, malvidin-3-O-galactoside chloride, peonidin-3-O-arabinoside chloride, malvidin-3-O-glucoside chloride, and malvidin-3-O-arabinoside chloride in the portion of Powdered Extract taken using the formula: 10,000(C/W)(rU / rS)in which C is the concentration, in mg per mL, of USP Cyanidin-3-O-glucoside Chloride RS in Standard solution 1; W is the weight, in mg, of Powdered Extract taken to prepare the Test solution; rU is the peak response obtained for each of the anthocyanosides in the Test solution; and rS is the peak response obtained for cyanidin-3-O-glucoside chloride in Standard solution 1. Add the percentages calculated for all the anthocyanosides: not less than 36.0% is found. Separately calculate the percentages of delphinidin chloride, cyanidin chloride, petunidin chloride, peonidin chloride, and malvidin chloride in the portion of Powdered Extract taken using the formula: 10,000(C/W)(rU / rS)in which C is the concentration, in mg per mL, of USP Cyanidin Chloride RS in Standard solution 2; W is the weight, in mg, of Powdered Extract taken to prepare the Test solution; rU is the peak response obtained for each of the anthocyanidins in the Test solution; and rS is the peak response obtained for cyanidin chloride in Standard solution 2. Add the percentages calculated for all the anthocyanidins: not more than 1.0% is found.

usp30-nf25 <711>dissolove 中有规定，参考里面的deslayed-relese dose forms 和extended-release dose forms项

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